PhenoPath’s R&D services include optimization and validation of immunohistochemistry (IHC), 10-color flow cytometry, fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR), RNA/DNA ISH, immunofluorescence (IF) and specialty pathology assays for use in biomarker discovery, drug development, diagnosis and clinical trial applications.
Human, animal, xenografts, and patient-derived xenografts are commonly used in our clinical studies. PhenoPath provides histology services for FFPE and frozen tissue, cell pellet and tissue microarray preparation, and special stains.
PhenoPath has developed and validated over 800 IHC assays in its clinical and CRO settings. Other validated assays include RNAScope, 10-color flow cytometry, FISH, DNA-based PCR, Real-Time-PCR, immunofluorescence, and cytogenetics.
PhenoPath pathologists, scientists, project managers, and all PhenoPath staff strive to create an environment of collaboration with our clients. Teleconferences or webinars with PhenoPath’s scientific team are available to optimize information exchange.
PhenoPath offers an extensive array of diagnostic assays including over 250 IHC antibodies validated in our clinical laboratory, an additional 100+ antibodies available in our research laboratory, over 100 flow cytometry antibodies, and a broad menu of FISH, PCR, cytogenetics and IF assays. Antibody targets include tissue-specific markers, tumor prognostic and predictive markers, surrogate markers of chromosomal translocations, and a broad range of esoteric antigens.
PhenoPath utilizes advanced analytic instrumentation and internally developed information management technology to ensure the highest quality results, rapid test turnaround, and streamlined data transfer.
|MetaSystems Metafer 4||FISH, Cytogenetics|
|Beckman Coulter GalliosTM||10-Color Flow Cytometry|
|Roche cobas z 480||PCR|
|Qiagen Rotor-Gene Q||PCR|
|Dako Autostainer Link 48||IHC|
|Dako Autostainer - other||IHC|
|Leica-BOND-MAX||IHC, RNA ISH|
|￼Aperio whole slide imaging||IHC|
|￼PerkinElmer Nuance/inForm Multispectral Imaging||IHC, IF|
Additional technologies may be available; please inquire.
PhenoPath provides assay feasibility, optimization and validation services, including the selection of appropriate reagents, positive and negative controls, and appropriate tissues. Validation protocols include testing for specificity, sensitivity and reproducibility. PhenoPath is experienced in technology transfers of client-validated procedures. At the culmination of client-specific validation activities, PhenoPath provides the client with a comprehensive validation report upon request.
PhenoPath’s team of pathologists, scientists, technicians, and project managers will help to research, design, and manage your project. PhenoPath’s experience with a wide variety of tissue types, methodologies and technologies provides a solid background for developing, validating and implementing each project.
Method Development (IHC):
Method Development (10-color flow cytometry):