Fibrillins in human adult ovary and polycystic ovary syndrome: is fibrillin-3 affected in PCOS?

By Jordan CD, Bohling SD, Charbonneau N, Sakai L.

J Histochem Cytochem 58(10): 903-15, 2010

Polycystic ovary syndrome (PCOS) is a common endocrinopathy in women of reproductive age. Although genetic linkage analyses have demonstrated a susceptibility locus for PCOS mapping to the fibrillin-3 gene, the presence of fibrillin proteins in normal and polycystic ovaries has not been characterized. This study compared and contrasted fibrillin-1, -2, and -3 localization in normal and polycystic ovaries. Immunohistochemical stainings of ovaries from 21 controls and 9 patients with PCOS were performed. Fibrillin-1 was ubiquitous in ovarian connective tissue. Fibrillin-2 localized around antral follicles and in areas of folliculolysis. Fibrillin-3 was present in a restricted distribution within the specialized perifollicular stroma of follicles in morphological transition from primordial to primary type [transitional follicles (TFs)]. Fibrillin-1 and -2 stainings of PCOS ovaries were similar to those of the controls. However, in eight of the nine PCOS ovaries, there was a decrease in the number of TFs associated with fibrillin-3, including no staining in five PCOS samples; decreased number of fibrillin-3-associated TFs/mm(2) was confirmed by quantitative analysis. Our findings support a role for fibrillin-3 in the pathogenesis of PCOS and suggest fibrillin-3 may function in primordial to primary follicle transition. We propose that loss of fibrillin-3 during folliculogenesis may be an important factor in PCOS pathogenesis.
PMID: 20855553 [PubMed - indexed for MEDLINE] 
PMCID: PMC2942743

View article on >


PhenoPath, PLLC

About PhenoPath


Medical Staff

Executive Staff

Licensing & Accreditation


Test Menu


Diagnostic Services

Clinical Setting

Educational Media

Contact Diagnostic Services

BioPharma Services

Clinical Trial Services

R & D Services



Contact BioPharma Services

News & Info

Company News



Upcoming Events

Billing Information

Contact Us

Dept. Inquiries

Submit a Case

Order Supplies


Patients & Clients