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Methodology: Flow Cytometry
This assay includes one tube with a cytoplasmic bcl-2 antibody and a second with an isotype control, and is added on to the workup virtually all CD10+ mature B cell lymphomas. Assessment of cytoplasmic bcl-2 allows distinction of BL (bcl-2 negative, and usually strongly CD38+) from other follicle center-derived B cell lymphomas usually bcl-2-positive, with highly variable CD38). Since CD11c is almost never expressed in FL or BL, if one detects a CD10+/CD11c+ B cell neoplasm, one should strongly consider CD10+ hairy cell leukemia, which is reported to represent ~15% of classic hairy cell leukemia. This tube includes antibodies to CD45, bcl-2, CD10, CD19, CD5, CD20, CD11c, and CD38.
Organ: Blood/Bone Marrow
Disease State: Burkitt's Lymphoma
CPT Code(s): 88185x8 (reference only; CPTs may vary)
Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media