Follicular lymphoma (FL)/Burkitt lymphoma (BL) add-on tubes pin

FLOW

Follicular lymphoma (FL)/Burkitt lymphoma (BL) add-on tubes

Panel Components:
bcl-2, CD5, CD10, CD11c, CD19, CD20, CD38, CD45

Methodology: FLOW

Clinical Significance:
This assay includes one tube with a cytoplasmic bcl-2 antibody and a second with an isotype control, and is added on to the workup virtually all CD10+ mature B cell lymphomas. Assessment of cytoplasmic bcl-2 allows distinction of BL (bcl-2 negative, and usually strongly CD38+) from other follicle center-derived B cell lymphomas usually bcl-2-positive, with highly variable CD38). Since CD11c is almost never expressed in FL or BL, if one detects a CD10+/CD11c+ B cell neoplasm, one should strongly consider CD10+ hairy cell leukemia, which is reported to represent ~15% of classic hairy cell leukemia. This tube includes antibodies to CD45, bcl-2, CD10, CD19, CD5, CD20, CD11c, and CD38.

Organ: Blood/Bone Marrow, Body Fluids, Lymph Node / Spleen

Disease State: Burkitt's Lymphoma, Follicular Dendritic Cell Sarcoma, Follicular Lymphoma

CPT Code(s): 88185x8 (reference only; CPTs may vary)

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR -
• Tissue: Finely minced tissue in RPMI transport media - OR -
• Body fluids: In RPMI transport media