Hydatidiform Mole Evaluation (Molar Pregnancy) pin

FISH

Hydatidiform Mole Evaluation (Molar Pregnancy)

Panel Components:
p57 and Ki-67 (MIB-1) by IHC
CEP17 by FISH
* Panel makeup subject to change

Methodology: FISH

Clinical Significance:
p57kip2 is a paternally imprinted, maternally expressed gene that is not expressed in the cytotrophoblasts of complete moles (which are of paternal origin only). It is positive in both partial moles and normal hydropic villi. In addition, complete moles are characterized by high Ki-67-defined proliferative index in the cytotrophoblasts.

Further distinction of partial moles from hydropic normal villi can be made by identifying the triploid nature of incomplete moles. This can be accomplished by fluorescence in situ hybridization studies using a chromosome 17 probe to ascertain ploidy. It should be noted that these mole ploidy studies are best interpreted in conjunction with morphology for the evaluation of both complete and partial moles.

Organ: OB/GYN

Disease State: Molar Pregnancy

CPT Code(s): 88342; 88360; 88367; 88323 (reference only; CPTs may vary)

Turnaround Time: Within 3-4 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Formalin-fixed, paraffin-embedded (FFPE) tissue block or cell block - OR -
• Minimum of 5 unstained slide cut at 4µm for each test requested plus 2 unstained slides cut at 6µm