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Immunofluorescence
Methodology: Immunofluorescence
Clinical Significance:
Identifies: IgM localization by direct immunofluorescence is useful in the subclassification of bullous and other skin disorders, particularly when employed as part of a panel of FITC-conjugated antibodies, including those to C3, IgG and IgA. IgM deposits in the skin, with unique patterns, are characteristic of specific dermatologic disorders, including vasculitis, lupus, and others.
Subcellular Localization: Extracellular
Organ: Skin & Mucosa
Disease State:
CPT Code(s): 88346 or 88350 (reference only; CPTs may vary)
Turnaround Time: Within 1-2 business days of receipt
Schedule: Monday - Friday
Specimen Requirements:
Skin biopsy in Michel's (aka Zeuss) fixative, with or without patient serum