Kappa Light Chains pin

IHC

Kappa Light Chains

Methodology: IHC

Clinical Significance:
Identifies: Kappa and lambda immunoglobulin light chains, expressed by plasma cells and plasmacytoid lymphocytes. Also expressed, but less reliably detected, on B cells with small amounts of cytoplasm. The ratio of kappa-positive to lambda-positive cells in normal/polyclonal B cell and plasma cell populations typically ranges from 1:1 to 2:1. Marked increases in this ratio suggest kappa light chain-restriction, while marked decreases in the ratio (significantly < 1) suggest lambda light chain-restriction. Light chain restriction is conventionally used as a surrogate for clonality among B cell neoplasms.

Subcellular Localization: Cytoplasmic

Organ:

Disease State: Amyloid Type Analysis, B Cell NHL with Plasmacytoid Differentiation, HIV-Associated Lymphoma, Post-Transplant Lymphoproliferative Disorders

CPT Code(s): 88342 or 88341 (reference only; CPTs may vary)

Turnaround Time: Within 1-2 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Formalin-fixed, paraffin-embedded (FFPE) tissue block or cell block - OR -
• 1 unstained slide for each test requested plus 3-4 additional unstained slides cut at 4µm

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