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Large T cell therapy add-on tube pin

Flow Cytometry

Large T cell therapy add-on tube

Methodology: Flow Cytometry

Clinical Significance:
This two-tube add-on is used when the population of interest shows large cell size by morphology and/or forward light scatter, and when evaluation of CD30 and CD25 expression is of interest. The base antibodies for these add-on tubes include CD45, CD52, CD30, CD3, CD25, and HLA-DR; additional T cell-associated antigens, such as CD5, CD4, and CD8, may be evaluated in this tube to aid in identification of the abnormal population.

Organ: Blood/Bone Marrow

Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous)

CPT Code(s): 88185x6 (reference only; CPTs may vary)

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media