MYC Breakapart pin

FISH

MYC Breakapart

Alternate Test Names:
c-MYC
Non Hodgkin Lymphoma (Agressive B-NHL) Panel test
MYC Panel test
ALL Panel test: MYC (8q24) rearrangements

Methodology: FISH

Clinical Significance:
This sensitive breakapart FISH assay detects any rearrangement involving the MYC gene. Translocations involving the MYC gene on chromosome 8 are characteristic chromosomal aberrations of Burkitt's-type lymphomas/leukemias (BLs). On the molecular level, the MYC gene on 8q24 is juxtaposed to the heavy chain locus on 14q32 in most cases or less commonly to light chain loci on 2q11 and 22q11, resulting in constitutive overexpression of the transcription factor, MYC. The detection of translocations involving MYC is a major aim in the diagnosis of patients with high-grade B cell lymphomas because treatment strategies differ between BL and other high-grade B cell neoplasms.

Probe type: Dual color breakapart

Organ: Blood/Bone Marrow, Body Fluids, Brain/CNS, Colon & Other GI, Lymph Node / Spleen

Disease State: B Cell Non-Hodgkin Lymphoma (not otherwise classified), Burkitt's Lymphoma, Diffuse Large B Cell Lymphoma

CPT Code(s): 88374 (reference only; CPTs may vary)

Turnaround Time: Within 3-4 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR -
• Fresh tissue in RPMI - OR -
• Formalin-fixed, paraffin-embedded tissue (FFPE)

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