Methodology: Flow Cytometry

Clinical Significance:
This tube is designed to help identify erythroid and megakaryocytic differentiation in the setting of AML, and also to help distinguish AML from blastic plasmacytoid dendritic cell neoplasms. The tube includes antibodies to CD45, CD235a (glycophorin A), CD41, CD34, CD117, CD61, and CD11c.

Organ: Blood/Bone Marrow

Disease State: Acute Myeloid Leukemia (AML) / Myeloid Sarcoma

CPT Code(s): 88185x7 (reference only; CPTs may vary)

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media