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Paroxysmal nocturnal hemoglobinuria (PNH) panel pin

Flow Cytometry

Paroxysmal nocturnal hemoglobinuria (PNH) panel

Methodology: Flow Cytometry

Clinical Significance:
Red blood cells are evaluated for deficiency of Gpi8 linkages using antibodies to CD235a/glycophorin A and CD59. The leukocytes are evaluated for loss of Gpi8 linkages using antibodies to CD45, CD33, HLA-DR (the latter two to help separate monocytes from granulocytes in gating), FLAER, CD24, CD14, and CD16. An antibody to CD10 is also present, to aid in the identification of mature neutrophils among the myeloid cells.

Organ: Blood/Bone Marrow

Disease State: Paroxysmal Nocturnal Hemoglobinuria (PNH)

CPT Code(s): 88184 x 1, 88185 x 9, 88188 x 1. The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
Ideally performed on peripheral blood; can be performed on bone marrow aspirate (less desirable than blood): • Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top)