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Methodology: Flow Cytometry
Red blood cells are evaluated for deficiency of Gpi8 linkages using antibodies to CD235a/glycophorin A and CD59. The leukocytes are evaluated for loss of Gpi8 linkages using antibodies to CD45, CD33, HLA-DR (the latter two to help separate monocytes from granulocytes in gating), FLAER, CD24, CD14, and CD16. An antibody to CD10 is also present, to aid in the identification of mature neutrophils among the myeloid cells.
Organ: Blood/Bone Marrow
Disease State: Paroxysmal Nocturnal Hemoglobinuria (PNH)
CPT Code(s): 88184 x 1, 88185 x 9, 88188 x 1 (reference only; CPTs may vary)
Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.
Ideally performed on peripheral blood; can be performed on bone marrow aspirate (less desirable than blood): • Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top)