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Methodology: Flow Cytometry
This two-tube assay includes one tube targeted primarily at mature B cells, and a second tube targeted primarily at mature T and NK cells. This panel is ideal for ruling out lymphoma in any type of fresh material. Both tubes contain nine antibodies (“9 colors”), with a tenth color allowing use of the DNA binding dye DAPI to facilitate exclusion of DAPI-positive dead and dying cells from the analysis. Antigens evaluated in the B cell tube include CD45, kappa, lambda, CD19, C5, CD20, CD10, CD56, and CD38. Antigens evaluated in the T cell tube include CD45, CD2, CD7, CD3, CD34, CD56, CD5, CD4, and CD8. Potential add-on tubes include: • Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL)/ mantle cell lymphoma (MCL) add-on tube • Follicular lymphoma (FL)/Burkitt lymphoma (BL) add-on tubes • Hairy cell leukemia/marginal zone lymphoma add-on tube • CD5-negative B cell therapy add-on tube • CD5-positive B cell therapy add-on tube • Rule out plasma cell dyscrasia tube • B cell versus plasma cell add-on tube • T cell receptor add-on tube • T cell therapy add-on tube • Large T cell therapy add-on tubes • B-ALL add-on tube • T-ALL add-on tube
Organ: Blood/Bone Marrow
Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous)
CPT Code(s): 88184 x 1, 88185 x 14, 88188 x 1. The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media