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Rule out lymphoma panel pin

Flow Cytometry

Rule out lymphoma panel

Methodology: Flow Cytometry

Clinical Significance:
This two-tube assay includes one tube targeted primarily at mature B cells, and a second tube targeted primarily at mature T and NK cells. This panel is ideal for ruling out lymphoma in any type of fresh material. Both tubes contain nine antibodies (“9 colors”), with a tenth color allowing use of the DNA binding dye DAPI to facilitate exclusion of DAPI-positive dead and dying cells from the analysis. Antigens evaluated in the B cell tube include CD45, kappa, lambda, CD19, C5, CD20, CD10, CD56, and CD38. Antigens evaluated in the T cell tube include CD45, CD2, CD7, CD3, CD34, CD56, CD5, CD4, and CD8. Potential add-on tubes include: • Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL)/ mantle cell lymphoma (MCL) add-on tube • Follicular lymphoma (FL)/Burkitt lymphoma (BL) add-on tubes • Hairy cell leukemia/marginal zone lymphoma add-on tube • CD5-negative B cell therapy add-on tube • CD5-positive B cell therapy add-on tube • Rule out plasma cell dyscrasia tube • B cell versus plasma cell add-on tube • T cell receptor add-on tube • T cell therapy add-on tube • Large T cell therapy add-on tubes • B-ALL add-on tube • T-ALL add-on tube

Organ: Blood/Bone Marrow

Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous)

CPT Code(s): 88184 x 1, 88185 x 14, 88188 x 1 (reference only; CPTs may vary)

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media