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Methodology: Flow Cytometry
This two-tube panel is designed to look for aberrant expression of CD25 and/or CD2 among the brightly CD117+ mast cells in the marrow. It is a two-tube panel because isotype controls are used for the CD2 and CD25 antibodies. The antibodies in these tubes include CD45, CD2, CD33, CD34, CD25, HLA-DR, CD117, CD4, and CD38.
Organ: Blood/Bone Marrow
Disease State: Mastocytosis
CPT Code(s): 88184 x 1, 88185 x 8, 88188 x 1 (reference only; CPTs may vary)
Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media