Methodology: Flow Cytometry

Clinical Significance:
This tube assesses TCR-alpha-beta and TCR-gamma-delta expression in the T cell population of interest, including looking for aberrant loss of TCR-alpha-beta in abnormal T cell populations. The baseline antibodies in this tube include CD45, TCR-gamma-delta, TCR-alpha-beta, CD3, CD56, CD4, CD5, and CD8.

Organ: Blood/Bone Marrow

Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous)

CPT Code(s): 88185x8 (reference only; CPTs may vary)

Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.

Schedule: Monday-Saturday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Tissue: Finely minced tissue in RPMI transport media - OR - • Body fluids: In RPMI transport media