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Flow Cytometry
Methodology: Flow Cytometry
Clinical Significance:
This tube assesses TCR-alpha-beta and TCR-gamma-delta expression in the T cell population of interest, including looking for aberrant loss of TCR-alpha-beta in abnormal T cell populations. The baseline antibodies in this tube include CD45, TCR-gamma-delta, TCR-alpha-beta, CD3, CD56, CD4, CD5, and CD8.
Organ: Blood/Bone Marrow
Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous)
CPT Code(s): 88185x8 (reference only; CPTs may vary)
Turnaround Time: Physician will be contacted by 9:00 PM PST the day of receipt for acute/life threatening conditions; next business day for routine flow cases.
Schedule: Monday-Saturday
Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR -
• Tissue: Finely minced tissue in RPMI transport media - OR -
• Body fluids: In RPMI transport media