t(11;14) CCND1/IGH pin

FISH

t(11;14) CCND1/IGH

Alternate Test Names:
t(11;14)(q13;q32) (IGH/CCND1)
Myeloma Panel test
CLL/SLL Panel test
Non Hodgkin Lymphoma (Indolent B-NHL) Panel test

Methodology: FISH

Clinical Significance:
This FISH assay detects the chromosomal translocation t(11;14)(q13;q32), which is present in over 95% of mantle cell lymphomas (MCL) and is the most common translocation in multiple myeloma, occurring in 15-25% of cases. This translocation juxtaposes the CCND1 gene with the IGH gene resulting in constitutive overexpression of cyclin D1 protein which leads to deregulated cell cycle control and ultimately tumor cell proliferation. Because of the aggressive nature of mantle cell lymphomas, it is important to identify these lymphomas and differentiate them from other small B cell lymphomas that coexpress CD5 (particularly chronic lymphocytic leukemia/small lymphocytic lymphoma). In addition, patients with multiple myeloma that have a t(11;14)(q13;q32) have been reported to have a neutral to slightly improved clinical course.

Probe type: Dual color, dual fusion translocation

Organ: Blood/Bone Marrow, Body Fluids, Colon & Other GI, Lymph Node / Spleen

Disease State: B Cell Non-Hodgkin Lymphoma (not otherwise classified), Chronic Lymphocytic Leukemia / Small Lymphocytic Lymphoma (CLL/SLL), Mantle Cell Lymphoma, Plasma Cell Myeloma/Neoplasm

CPT Code(s): 88374 (reference only; CPTs may vary)

Turnaround Time: Within 3-4 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR -
• Fresh tissue in RPMI - OR -
• Formalin-fixed, paraffin-embedded tissue (FFPE)

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