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This FISH assay detects the chromosomal translocation t(11;14)(q13;q32), which is present in over 95% of mantle cell lymphomas (MCL) and is the most common translocation in multiple myeloma, occurring in 15-25% of cases. This translocation juxtaposes the CCND1 gene with the IGH gene resulting in constitutive overexpression of cyclin D1 protein which leads to deregulated cell cycle control and ultimately tumor cell proliferation. Because of the aggressive nature of mantle cell lymphomas, it is important to identify these lymphomas and differentiate them from other small B cell lymphomas that coexpress CD5 (particularly chronic lymphocytic leukemia/small lymphocytic lymphoma). In addition, patients with multiple myeloma that have a t(11;14)(q13;q32) have been reported to have a neutral to slightly improved clinical course. Probe type: Dual color, dual fusion translocation
Organ: Blood/Bone Marrow
Disease State: B Cell Non-Hodgkin Lymphoma (not otherwise classified)
CPT Code(s): 88374 (reference only; CPTs may vary)
Turnaround Time: Within 4-6 business days of receipt
Schedule: Monday - Friday
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR - • Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR - • Fresh tissue in RPMI - OR - • Formalin-fixed, paraffin-embedded tissue (FFPE)