t(4;14) FGFR3/IGH pin

FISH

t(4;14) FGFR3/IGH

Alternate Test Names:
t(4;14)(p16;q32) (IGH/FGFR3)
Myeloma Panel test

Methodology: FISH

Clinical Significance:
This FISH assay detects the t(4;14)(p16;q32) which juxtaposes the FGFR3 gene with the IGH gene. This translocation has been identified as a recurrent and prognostic translocation in plasma cell myeloma and has been associated with a poor prognosis with shorter overall survival. Plasma cell myeloma is a bone marrow-based plasma cell proliferation characterized by elevated circulating monoclonal paraprotein production and multifocal osteolytic bone lesions. Separation of plasma cell myeloma into different subgroups based upon recurrent chromosomal alterations may provide important prognostic information and may provide the basis for particular therapy for some plasma cell myeloma subgroups.

Probe type: Dual color, dual fusion translocation

Organ: Blood/Bone Marrow, Body Fluids, Brain/CNS

Disease State: Plasma Cell Myeloma/Neoplasm

CPT Code(s): 88374 (reference only; CPTs may vary)

Turnaround Time: Within 3-4 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Peripheral blood, Preferred: 3 ml in sodium heparin (green top) / Acceptable: 3 ml in EDTA (purple top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in sodium heparin (green top) / Acceptable: 1-2 ml in EDTA (purple top) - OR -
• Fresh tissue in RPMI - OR -
• Formalin-fixed, paraffin-embedded tissue (FFPE)

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