We are proud to announce that PhenoPath is now a Quest Diagnostics Company.

TCR-gamma (T-cell) gene rearrangement pin


TCR-gamma (T-cell) gene rearrangement

Alternate Test Names:
T cell clonality

Methodology: PCR

Clinical Significance:
The TCR-gamma Clonality Assay is a multiplex fluorescent PCR assay based on the pan-European collaborative study (BIOMED-2). This assay uses the Vgamma 1f/10 and Vgamma 9/11 primer sets utilized by BIOMED-2 for the analysis of TCR gamma gene rearrangements. Importantly, this test should be interpreted in the context of clinical, histologic, and immunophenotypic information and should not be used in isolation in the evaluation of possible T cell lymphoproliferative disorders. Clinical indications for the test include:

• Any suspect T cell proliferation when morphology and immunophenotyping are not conclusive, particularly in atypical proliferations in the skin
• Lymphoproliferations in immunodeficient patients
• Evaluation of the clonal relationship between two T lymphoid malignancies in one patient or discrimination between a relapse and a second malignancy
• Occasionally, staging of T cell lymphomas
• Aid in minimum residual disease detection

Type: PCR/Capillary Electrophoresis, Qualitative

Organ: Skin & Mucosa

Disease State: Anaplastic Large Cell Lymphoma (Systemic or Cutaneous), Angioimmunoblastic T Cell Lymphoma, NK/T Cell Lymphoma, Nasal Type, Precursor T-Lymphoblastic Lymphoma/Leukemia (T-ALL), T Cell Non-Hodgkin Lymphoma (not otherwise classified)

CPT Code(s): 81342; 88381 may apply; G0452 may apply (reference only; CPTs may vary)

Turnaround Time: Within 3-5 business days of receipt

Schedule: Monday - Friday

Specimen Requirements:
• Peripheral blood, Preferred: 5 ml in EDTA (purple top) / Acceptable: 5 ml in sodium heparin (green top) - OR -
• Bone marrow aspirate, Preferred: 1-2 ml in EDTA (purple top) / Acceptable: 1-2 ml in sodium heparin (green top) - OR -
• Fresh tissue in RPMI - OR -
• Formalin-fixed, paraffin-embedded tissue (FFPE)